2. What are the practical applications of DNA technology?
3. What is a gel electrophoresis?
1. Dna clonning manipulate and analyze dna and produce useful new products and organisms.
The cloning of genes occurs in five steps. identify and isolate the gene of interest and a cloning vector. cut both gene of interest and the vector with the same restriction enzyme. join the two pieces of dna. get the vector carrying the gene of interest into a host cell.
Video on Gene Clonning:
2. Diagnosis of disease, gene therapy, the production of pharmaceuticals, forensic applications, environmental cleanup, agricultural applications.
Gene therapy image:
3. Gel electrophoresis is a one indirect method of rapidly analyzing and comparing genomes. it separtes macromolecules on the basis of their rate of movement through a gel in an electrical field.
5 facts about this chapter:
. The major goal of most animal cloning is reproduction, but not for humans. in humans, the major goal is to produce stem cells.
. When a dna molecule is cut by restriction enzymes, the result will always be a set of restriction fragments, which will have at least one single-stranded end, called sticky end.
. A genomic library is a set of thousands of recombinant plasmid clones, each of which has a piece of the original genome being studied.
. PCR is a method used to greatly amplify a particular piece of dna without the use of cells.
. a cDNA library is made up of complementary dna made from mRNA transcribed by reverse transcriptase. this technique rids th gene of introns but may not contain every gene in the organism.
This chapter is quiet complicated for me. I dont think I understood the whole concept.
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